Chalk N Duster is the emotional journey of two teachers Vidya (Shabana Azmi) and Jyoti (Juhi Chawla), serving at a Mumbai-based high school. Their passion and love for teaching bonds them in a special relationship with their students. Their gratification is good grades, moral values they strive to impart to students. However, in an evil turn of events, Kamini Gupta (Divya Dutta) is appointed as School Principal. Cunning by nature, she exploits every opportunity to make profits for the school and decides to replace experienced teachers with fresh teachers, thus affecting the quality of the school`s education. Vidya and Jyoti fight together to earn their respect and credibility as teachers and unite against prejudices set by the management.