A prolific theater artist Ayesha Raza Mishra is seldom seen working in movies. Since two of her grand-aunts were theater actors, Ayesha had always been exposed to theater. She soon took to performing on stage after training under the guidance of Amal and Nissar Allana at the Dramatic Art and Design Academy in New Delhi. She has also been a part of two very popular YouTube web series Permanent Roommates and Bang Baaja Baarat. With several plays to her credit, Ayesha has also appeared in movies such as Dhoom, Happy Bhaag Jayegi, Dil Dhadakne Do, Befikre, Toilet - EK Prem Katha and Sonu Ke Titu Ki Sweety. Her 2020 releases include Ginny Weds Sunny and Laxmii.

Ayesha Raza Mishra continued her work as an Actor in 2022 with the comedy-drama Sharmaji Namkeen (2022). In 2023, she appeared as an Actor in the family drama The Great Indian Family (2023) and the comedy Khichdi 2: Mission Paanthukistan (2023). Her work in 2026 includes her role as an Actor in the romantic drama Do Deewane Seher Mein (2026).