An Indian actor, Mayilsamy is best known for his supporting and comic roles in Tamil films such as Dhool, Gilli, Kangalal Kaidhu Sei, Devathayai Kanden, Rendu and Thiruvilaiyaadal Aarambam.

Mayilsamy, also credited as R. Mayilsamy, started his career as a theatre and drama artist. Meanwhile, he was also a part of popular comedy troupe named Sirrippu Isai, where he was spotted by the Tamil filmmakers. The actor made his silver screen debut in the 1985 Tamil film Kanni Rasi. Since then, the actor has done over 100 Tamil films, playing a variety of roles. He has also played supporting roles in countless Tamil films such as Aboorva Sagodharargal, Sivalingam, Nade Namade, Vetri Raagangal, Villathi Villain and many more.

Known for his excellent comic timing, Mayilsamy gained popularity partnering with comedian Vivek in various films such as Dhool, Kandaen Seethayei and Prabhu Deva and Jaya Seal starrer Penin Manathai Thottu. The actor-comedian has worked in films such as En Purushan Kuzhandhai Maathiri, Bala, Military, Kannadi Pookal, Machakaaran, and Kadhal. His other projects include Jithan 2, Podhuvaga En Manasu Thangam, Server Sundaram, Yaamirukka Bayamey, Enna Thavam Seitheno and Annanukku Jey. His 2019 releases include Kolaigaran and Nenjamundu Nermaiyundu Odu Raja