Seema Deshmukh has been a professional actress for the past 35 years. She has worked in several Marathi and Hindi television shows, Marathi films, and has a strong background in both parallel and commercial Marathi theatre.

She is known for television shows such as Asambhav, Saath Nibhaana Saathiya, and Maza Hoshil Na. Her Marathi film credits include Vis, Nirop, TTMM, Time Please, Mala Kahich Problem Nahi, Ek Nirnay: Swatahcha Swatasathi, and Jaarann. Her other film credits include Salaam Bacche, Gandha, Dham Dhoom, Tujha Tu Majha Mi, AB Aani CD, and Befaam.

Her theatre credits include Indu Kale Sarala Bhole, Naandi, Alibaba Ani Chalishitale Chor, and Ashrunchi Zali Phule. She is currently associated with the play Shubhmkaroti.